Publications


Publications

Click on reference number for direct link to PubMed !

 1. Johnston, L.J., A.J. Thulin, W.C. Weldon, O.A. MacDougald, and E.R. Miller. 1988. Carbadox-Pyrantel Tartrate Combination and Elevated Dietary Protein: Effects on Pig Performance and Body Composition. Nutrition Reports International. 38: 799 - 804.

2. MacDougald, O.A., A.J. Thulin, W.C. Weldon, J.J. Pestka, and R.L. Fogwell. 1990. Effects of Immunizing Gilts Against Zearalenone on Height of Vaginal Epithelium and Urinary Excretion of Zearalenone. Journal of Animal Science. 68: 3713 - 3718.

3. MacDougald, O.A., A.J. Thulin, and J.J. Pestka. 1990. Determination of Zearalenone and Related Metabolites in Porcine Urine by Modified Enzyme-Linked Immunosorbent Assay. Journal of the Association of Official Analytical Chemists. 73: 65 - 68.

4. Weldon, W.C., A.J. Thulin, O.A. MacDougald, L.J. Johnston, E.R. Miller, and H.A. Tucker. 1991. Effects of Elevated Energy and Protein during Late Gestation on Mammary Development in Gilts. Journal of Animal Science. 69: 194 - 200.

5. MacDougald, O.A. and D.B. Jump. 1991. Identification of Functional Cis-Acting Elements Within the Rat Liver S14 Promoter. Biochemical Journal. 280: 761 - 767.

6. MacDougald, O.A., S.D. Clarke, and D.B. Jump. 1992. Tissue-Specificity of S14 and Fatty Acid Synthase In Vitro Transcription. Biochemical and Biophysical Research Communications. 182: 631 - 637.

7. MacDougald, O.A. and D.B. Jump. 1992. Localization of an Adipocyte-Specific Retinoic Acid Response Domain Controlling S14 Gene Transcription. Biochemical and Biophysical Research Communications. 188: 470 - 476.

8. Jump, D.B. and O.A. MacDougald. 1993. Hormonal Regulation of Gene Expression in Cultured Adipocytes. Journal of Animal Science. 71(Suppl. 2): 56-64.

9. Jump, D.B., S.D. Clarke, O.A. MacDougald, and A. Thelen. 1993. Polyunsaturated Fatty Acids Inhibit S14 Gene Transcription in Rat Liver and Cultured Hepatocytes. Proceedings of the National Academy of Sciences, USA. 90: 8454 - 8458.

10. Lin, F-T., O. A. MacDougald, A.M. Diehl, and M.D. Lane. 1993. A 30-kDa Alternative Translation Product of the CCAAT/Enhancer Binding Proteina message: Transcriptional Activator lacking Antimitotic Activity. Proceedings of the National Academy of Sciences, USA. 90: 9606-9610.

11. Landschulz, K.T., D.B Jump, O.A. MacDougald, and M.D. Lane. 1994. Transcriptional Control of the Stearoyl-CoA Desaturase-1 Gene by Polyunsaturated Fatty Acids. Biochemical Biophysical Research Communications. 200: 763-768.

12. MacDougald, O.A., P. Cornelius, F.-T. Lin, S.S. Chen, and M.D. Lane. 1994. Glucocorticoids Reciprocally Regulate Expression of the CCAAT/Enhancer-binding Protein a and d Genes in 3T3-L1 Adipocytes and White Adipose Tissue. Journal of Biological Chemistry. 269: 19041-19047.

13. MacDougald, O.A., P. Cornelius, and M.D. Lane. 1995. Insulin Regulates Transcription of the CCAAT/Enhancer Binding Protein (C/EBP) a, b and d Genes in Fully-Differentiated 3T3-L1 Adipocytes. Journal of Biological Chemistry. 270: 647-654.

14. Potter, J.J., O.A. MacDougald, and E. Mezey. 1995. Regulation of Rat Alcohol Dehydrogenase by Cyclic AMP in Primary Hepatocyte Culture. Archives of Biochemistry and Biophysics. 321: 329-335.

15. MacDougald, O.A., Hwang, C. Fan, H. and M.D. Lane. 1995. Regulated Expression of the obese Gene Product (Leptin) in White Adipose Tissue and 3T3-L1 Adipocytes. Proceedings of the National Academy of Sciences, USA. 92: 9034-9037.

16. Hwang, C.S., Mandrup, S., MacDougald, O.A., Geiman, D.E. and M.D. Lane. 1996. Transcriptional Activation of the Obese Gene by CCAAT/Enhancer Binding Protein a. Proceedings of the National Academy of Sciences. USA. 93: 873-877.

17. Mandrup, S., T. Loftus, O.A. MacDougald, F. Kuhajda, and M.D. Lane. 1997. Obese Gene Expression at In Vivo Levels by Fat Pads Derived from s.c. Implanted 3T3-F442A Preadipocytes. Proceedings of the National Academy of Sciences. U.S.A. 94: 4300-4305.

18. Hemati, N., S.E. Ross, R.L. Erickson, G.E. Groblewski, and O.A. MacDougald. 1997. Signaling Pathways through which Insulin regulates CCAAT/Enhancer Binding Protein a (C/EBPa) Phosphorylation and Gene Expression in 3T3-L1 Adipocytes: Correlation with GLUT4 Gene Expression. Journal of Biological Chemistry. 272: 25913-25919.

19. Hemati, N., R.L. Erickson, S.E. Ross, R. Liu, and O.A. MacDougald. 1998. Regulation of CCAAT/Enhancer Binding Protein a (C/EBPa) Gene Expression by Thiazolidinediones in 3T3-L1 Adipocytes. Biochemical Biophysical Research Communications. 244: 20-25.

20. Becker, D.J. and O.A. MacDougald. 1999. Transfection of 3T3-L1 Adipocytes by Gene Gun-Mediated Transfer. BioTechniques. 26: 660-668.

21. Liao, J., G. Piwien de Pilipuk, S.E. Ross, C. L. Hodge, L. Seally, O.A. MacDougald, and J. Schwartz. 1999. C/EBPb Contributes to GH-Regulated Transcription of c-Fos. Journal of Biological Chemistry. 274: 31597-31604.

22. Ross, S.E., R.L. Erickson, N. Hemati, and O.A. MacDougald. 1999. Glycogen Synthase Kinase 3 is an Insulin-Regulated C/EBPa Kinase. Molecular and Cellular Biology 19: 8433-8441.

23. Hajra, A.K., A.K. Das, L.K. Larkins, N. Hemati, R.L. Erickson, and O.A. MacDougald. 2000. Induction of the Peroxisomal Glycerolipid-synthesizing Enzymes during Differentiation of 3T3-L1 Adipocytes: Role in Triacylglycerol Synthesis. Journal of Biological Chemistry. 275: 9441-9446.

24. Ross, S.E., N. Hemati, K.A. Longo, C. Bennet, P. Lucas, and R.L. Erickson, O.A. MacDougald. 2000. Inhibition of Adipogenesis by Wnt Signaling. Science 289: 950-953.

25. Erickson, R.L., N Hemati, S.E. Ross, and O. A. MacDougald. 2001. p300 Coactivates the Adipogenic Transcription Factor C/EBPa. Journal of Biological Chemistry. 276: 16348-16355.

26. Piwien-Pilipuk, G., D. van Mater, S.E. Ross, O.A. MacDougald, and J. Schwartz. 2001. Growth hormone regulates phosphorylation and function of CCAAT/enhancer-binding protein beta by modulating Akt and glycogen synthase kinase-3. Journal of Biological Chemistry 2001June01; 276(22):19664-71. Epub 2001March13.

27. Schaufele, F., J.F. Enwright, III, X. Wang, C. Teoh, R. Srihari, R. Erickson, O.A. MacDougald, and R.N. Day. 2001. CCAAT/enhancer binding protein a assembles essential cooperating factors in common subnuclear domains. Molecular Endocrinology 15: 1665-1676.

28. Douglas, K.R., M.L. Brinkmeier, J.A. Kennell, P. Eswara, T.A. Harrison, A.I. Patrianakos, B.S. Sprecher, M.A. Potok, R.H. Lyons, Jr., O.A. MacDougald, and S.A. Camper. 2001. Identification of Members of the Wnt Signaling Pathway in the Embryonic Pituitary Gland. Mammalian Genome 12: 843-851.

29. You, Z., Z. Zhang, D. Saims, S. Chen, A.M. Brown, K.-L. Guan, O.A. MacDougald, G.Evans, J. Kitajewski, and C.-Y. Wang. 2002. Transcriptional Control of Apoptosis by Wnt Signaling: Induction of Cox-2 and WISP-1 to Suppress c-myc-mediated Apoptosis. Journal of Cell Biology 157: 429-440.

30. Piwien-Pilipuk G, MacDougald O, Schwartz J. Dual regulation of phosphorylation and dephosphorylation of C/EBPbeta modulate its transcriptional activation and DNA binding in response to growth hormone. J Biol Chem. 2002 Nov 15;277(46):44557-65. Epub 2002 Sep 3.

31. Ross, S.E., R.L. Erickson, I. Gerin, P.M. DeRose, L. Bajnok, K.A. Longo, D.E. Misek, R. Kuick, S.M. Hanash, K.B. Atkins, S. Mahle, H.I. Nebb, L. Madsen, K. Kristiansen, and O.A. MacDougald. 2002. Microarray analyses during adipogenesis: understanding the effects of Wnt-signaling on adipogenesis and the roles of LXR in adipocyte metabolism. Molecular and Cellular Biology 22: 5989-5999.

32. Bennett, C.N., S.E. Ross, K.A. Longo, L. Bajnok, N. Hemati, K.W. Johnson, S.D. Harrison, and O.A. MacDougald. 2002. Regulation of Wnt signaling during adipogenesis. Journal of Biological Chemistry 227(34):30998-1004.

33. Longo, K.A., J.A. Kennell, M.J. Ochocinska, S.E. Ross, W.S. Wright, and O.A. MacDougald. 2002. Wnt signaling protects 3T3-L1 preadipocytes from apoptosis through induction of insulin-like growth factors. Journal of Biological Chemistry
277(41):38239-44.

34. Bennett, C.N., C. Hodge, O.A. MacDougald, and J. Schwartz. 2003. Role of Wnt10b and C/EBPa in Spontaneous Adipogenesis of 243 Cells. Biochemical and Biophysical Research Communications 302: 12-16.

35. Kennell, J.A., E.E. O’Leary, B.M. Gummow, Gary D. Hammer, and O.A. MacDougald. 2003. TCF-4N, a novel isoform of mouse TCF-4, synergizes with b-catenin to coactivate C/EBPa and SF-1 transcription factors. Molecular and Cellular Biology 23: 5366-5375.

36. Ross, S.E., H.S. Radomska, F. Schaufele, P. Zhang, J.N. Winnay, L. Bajnok, W.S. Wright, D.G. Tenen and O.A. MacDougald. 2004. Phosphorylation of C/EBP inhibits granulopoiesis. Molecular and Cellular Biology 24:675-686 .

37. Longo, K.A., W.S. Wright, S. Kang, I. Gerin, S.-H. Chiang, P.C. Lucas, M.R. Opp, and O.A. MacDougald. 2004. Wnt10b Inhibits Development of White and Brown Adipose Tissues. Journal of Biological Chemistry 279: 35503-35509.

38. Vertino, A.M., J.M. Taylor-Jones, K.A. Longo, E.D. Bearden, T.F. Lane, R.E. McGehee, Jr., O.A. MacDougald, and C.A. Peterson. 2005 Wnt10b deficiency promotes coexpression of myogenic and adipogenic programs in myoblasts. Molecular Biology of the Cell. 16: 2039-2048.

39. Kang, S., L. Bajnok, K.A. Longo, R.K. Petersen, J.B. Hansen, K. Kristiansen, O.A. MacDougald. 2005. Effects of Wnt signaling on brown adipocyte differentiation and metabolism mediated by PGC-1a. Molecular and Cellular Biology 25: 1272-1282.

40. Gerin, I., V.W. Dolinsky, J.G. Shackman, R.T. Kennedy, S.-H. Chiang, C.F. Burant, K. Steffensen, J-Ä.Gustafsson, and O.A. MacDougald. 2005. LXRb is required for adipocyte growth, glucose homeostasis and b cell function in aged mice. Journal of Biological Chemistry 280: 23024-23031.

41. Bennett, C.N., K.A. Longo, W.S. Wright, L.J. Suva, T.F. Lane, K.D. Hankenson and O.A. MacDougald. 2005. Regulation of osteoblastogenesis and bone mass by Wnt10b. Proceedings of the National Academy of Sciences 102: 3324-3329.

42. Kennell, J.A. and O.A. MacDougald. 2005. Wnt signaling inhibits adipogenesis through beta-catenin-dependent and -independent mechanisms. Journal of Biological Chemistry 25: 24004-24010.

43. Xu, Y., Y.L. Zhou, D.K Ann, O.A. MacDougald, L. Shum, M.L. and Snead. 2006. Transcription factor sumoylation and factor YY1 serve to modulate mouse amelogenin gene expression. European Journal of Oral Sciences 114: 201-202.

44. Xu, Y., Y.L. Zhou, W. Luo, Q.S Zhu, D. Levy, O.A. MacDougald, and M.L. Snead. 2006. NF-Y and CCAAT/enhancer-binding protein alpha synergistically activate the mouse amelogenin gene. The Journal of Biological Chemistry 281: 16090-16098.

45. Inoki, K. , H. Ouyang, T. Zhu, C. Lindvall, Y. Wang, X. Zhang, Q. Yang, C. Bennett, Y. Harada, K. Stankunas, C.Y. Wang, X. He, O.A. MacDougald, M. You, B.O. Williams, and K.L. Guan. 2006. TSC2 integrates Wnt and energy signals via a coordinated phosphorylation by AMPK and GSK3 to regulate cell growth. Cell 126: 955-968.

46. Qiao, L., O.A. MacDougald, and J. Shao. 2006. CCAAT/enhancer-binding protein alpha mediates induction of hepatic phosphoenolpyruvate carboxykinase by p38 mitogen-activated protein kinase. The Journal of Biological Chemistry 281: 24390-24397.

47. Wright, W.S., K.A. Longo, V.W. Dolinsky, I. Gerin, S. Kang, C.N. Bennett, S.H. Chiang, T.C. Prestwich, C. Gress, C.F. Burant, V.S. Susulic, and O.A. MacDougald. 2007. Wnt10b inhibits obesity in ob/ob and agouti mice. Diabetes 56: 295-303.

48. Xu, Y. Y.L. Zhou, O.A. MacDougald, and M.L. Snead. 2007. Physical dissection of the CCAAT/enhancer-binding protein a in regulating the mouse amelogenin gene. Biochemical and Biophysical Research Communications 354:56-61.

49. Datta, J. S. Majumder, H. Kutay, W. Frankel, R. Costa, H.C. Cha, O.A. MacDougald, S.T. Jacob, and K. Ghoshal. 2007. Metallothionein expression is suppressed in primary human hepatocellular carcinomas and is mediated through inactivation of C/EBPa by phosphatidyl inositol 3 kinase signaling cascade.  Cancer Research 67: 2736-46.

50. Kang, S., C.N. Bennett, L.A. Rapp, K.D. Hankenson, and O.A. MacDougald. 2007. Wnt signaling stimulates osteoblastogenesis of mesenchymal precursors by suppressing C/EBPα and PPARγ. Journal of Biological Chemistry 282: 14515-24.

51. Bennett, C.N., H. Ouyang, Y. Ma, I. Gerin, K. Sousa, Q. Zeng, T.F. Lane, V. Krishnan, K.D. Hankenson, and O.A. MacDougald. 2007. Expression of Wnt10b increases postnatal bone formation by enhancing osteoblast differentiation. Journal of Bone and Mineral Research 22: 1924-1932.

52. Palpant, N.J., S.-I. Yasuda, O.A. MacDougald, and J.M. Metzger. 2007. Stromal vascular cells from mouse adipose tissue differentiate structurally and functionally into spontaneously beating myocytes containing developmental cardiac sarcomeric markers. Journal of Molecular and Cellular Cardiology 43: 362-370.

53. Aslanidi, G., G. Philipsberg, V. Kroutov, K. Lamb, E. Kohlbrenner, M. Campbell-Thompson, G. Walter, S. Kourenov, J.I. Aguirre, P. Keller, K.D. Hankenson, O.A. MacDougald, and S. Zolotukhin. 2007. Ectopic expression of Wnt10b decreases adiposity and improves glucose homeostasis in obese rats. American Journal of Physiology 293: E726-E736.

54. Bommer, G.T., I. Gerin, Y. Feng, A.J. Kaczorowski, R. Kuick, R.E. Love, Y. Zhai, T.J. Giordano, Z.S. Qin, B.B. Moore, O.A. MacDougald, K.R. Cho, E.R. Fearon. 2007. p53-Mediated activation of miRNA34 candidate tumor suppressor genes. Current Biology 17: 1298-307.

55. Huang, X., R.A. Charbeneau, Y. Fu, I. Kaur, I. Gerin, O.A. MacDougald, R.R. Neubig. 2008. Resistance to diet-induced obesity and improved insulin sensitivity in mice with an RGS insensitive G184S Gnai2 allele. Diabetes 57: 77-85.

56. Keller, P., J. Petrie, P. de Rose, I. Gerin, W.S. Wright, A. Rinnov, C.P. Fischer, B.K. Pedersen, and O.A. MacDougald. 2008. Fat specific protein 27 regulates storage of triacylglycerol. Journal of Biological Chemistry 283: 14355-65.

57. Cha, H.C., N.R. Oak, S. Kang, T.-A. Tran, S. Kobayashi, S.-H. Chiang, D.G. Tenen, and O.A. MacDougald. 2008. Phosphorylation of C/EBPα regulates GLUT4 expression and glucose transport in adipocytes. Journal of Biological Chemistry. Journal of Biological Chemistry 2008June27; 283 (26):18002-11. Epub 2008April11.

58. Park, K.W., Waki, H., Villanueva, C.J., Monticelli, .LA., Hong, C., Kang S, MacDougald, O.A., Goldrath, A.W., Tontonoz P. Inhibitor of DNA binding 2 is a small molecule-inducible modulator of peroxisome proliferator-activated receptor-gamma expression and adipocyte differentiation. Mol Endocrinol. 2008 Sep;22(9):2038-48. Epub 2008 Jun 18.

59. Luo, W., Shitaye, H., Friedma,n M., Bennett, C.N., Miller, J., Macdougald, O.A., Hankenson, K.D. Disruption of cell-matrix interactions by heparin enhances mesenchymal progenitor adipocyte differentiation. Exp Cell Res. 2008 Nov 1;314(18):3382-91. Epub 2008 Jul 12.

60. Kennell, J.A., Gerin, I., MacDougald, O.A., Cadigan, K,M. The microRNA miR-8 is a conserved negative regulator of Wnt signaling. Proc Natl Acad Sci U S A. 2008 Oct 7;105(40):15417-22. Epub 2008 Sep 29.

61. Fox, K.E., Colton, L.A., Erickson, P.F., Friedman, J.E., Cha, H.C., Keller, P., MacDougald, O.A., Klemm, D.J. Regulation of cyclin D1 and Wnt10b gene expression by cAMP-responsive element-binding protein during early adipogenesis involves differential promoter methylation. J Biol Chem. 2008 Dec 12;283(50):35096-105. Epub 2008 Oct 27.

62. Gerin, I., Louis, G.W., Zhang, X., Prestwich, T.C., Kumar, T.R., Myers, M.G., Jr., Macdougald, O.A., Nothnick, W.B. Hyperphagia and obesity in female mice lacking tissue inhibitor of metalloproteinase-1. Endocrinology. 2009 Apr;150(4):1697-704. Epub 2008 Nov 26.

63. Clark, A.M., Sousa, K.M., Jennings, C., Macdougald, O.A., Kennedy, R.T. Continuous-flow enzyme assay on a microfluidic chip for monitoring glycerol secretion from cultured adipocytes. Anal Chem. 2009 Mar 15;81(6):2350-6.

64. Gerin, I., Bommer, G.T., Lidell, M.E., Cederberg, A., Enerback, S., Macdougald, O.A. On the Role of FOX Transcription Factors in Adipocyte Differentiation and Insulin-stimulated Glucose Uptake. J Biol Chem. 2009 Apr 17;284(16):10755-63. Epub 2009 Feb 24.

65. Mor,i H., Inoki, K., Münzberg, H., Opland, D., Faouzi, M., Villanueva, E.C., Ikenoue, T., Kwiatkowski, D., MacDougald, O.A., Myers, M.G. Jr., Guan, K.L. Critical role for hypothalamic mTOR activity in energy balance. Cell Metab. 2009 Apr;9(4):362-74.

 

Reviews, Book chapters, New & Views, and Symposium Proceedings

1.  Jump, D.B., G.J. Lepar, and O.A. MacDougald. 1992. Retinoic Acid Regulation of Gene Expression in Adipocytes.  In:  Nutrient Control of Gene Expression,  (Eds. Berdiener, C. and J.L. Hargrove), CRC Press, Boca Raton, FL, pp 431-454.

2.  Cornelius, P., O.A. MacDougald, and M.D. Lane. 1994. Regulation of Adipocyte Development.  Annual Reviews of Nutrition. 14: 99-129.

3.  MacDougald, O.A. and M.D. Lane. 1995.   Transcriptional Regulation of Gene Expression During Adipocyte Differentiation.  Annual Reviews of Biochemistry. 64: 345-373.

4.  MacDougald, O.A. and M.D. Lane. 1995.  When Precursors are also Regulators. Current Biology. 5: 618-621.

5.  Lane, M.D., F.-T. Lin, O.A. MacDougald, and M.Vasseur-Cognet.  1996. Control of Adipocyte Differentiation by CCAAT/Enhancer Binding Proteina (C/EBPa). International Journal of Obesity and Related Metabolic Disorders. 20: S91-6 (Suppl. 3).

6. Darlington, G., S.E. Ross, and O.A. MacDougald. 1998. The Role of C/EBP Genes in Adipose Differentiation. Journal of Biological Chemistry. 273: 30057-30060.

7. Erickson, R.L., S.E. Ross, K.A. Longo, N. Hemati, and O.A. MacDougald. 2000. Structure and Function of C/EBPa. In Adipocyte Biology and Hormone Signaling (J.M. Ntambi; Ed.) IOS Press. pg. 79-90.

8.  MacDougald, O.A. 2001. Signaling Pathway Puts the Break on Fat Cell Formation. TheScientificWorld. 1: 188-189.

9.  MacDougald, O.A. and S. Mandrup. 2002. Adipogenesis: Forces that Tip the Scales. Trends in Endocrinology and Metabolism 13: 5-11.

10.  Longo, K.A. and O.A. MacDougald. 2003. Wnt signaling in regulation of adipogenesis. In: Progress in Obesity Research: 9. Edited by G. Medeiros-Neto, A. Halpern and C. Bouchard.  pp. 157-160.

11. Chiang, S.-H. and O.A. MacDougald. 2003. Will fatty worms help cure human obesity? Trends in Genetics 19: 523-525.

12. MacDougald, O.A. and C.F. Burant. 2005.  News and Views: Fickle Factor Foils Fat Fate. Nature Cell Biology 7: 543-545.

13. MacDougald, O.A. and C.F. Burant. 2005. Commentary: Obesity and metabolic perturbations following loss of aquaporin 7, the adipose glycerol transporter. Proceedings of the National Academy of Sciences 102: 10759-10760.

14. Hall, C.L., S. Kang, O.A. MacDougald, and E.T. Keller.  2006. The role of Wnts in bone metastasis. J. Cellular Biochemistry 97: 661-672.

15. Gerin, I., H.C. Cha, and O.A. MacDougald. 2006. Regulation of adipocyte differentiation and metabolism by Wnt signaling and C/EBP transcription factors. In: New Transcription factors and their Roles in Diabetes and its Therapy. (J.E. Friedman, ed.), Advances in Molecular and Cellular Endocrinology 5:155-174. Elsevier, San Diego, CA.

16. Venkatesh, J., H.U. Bryant, and O.A. MacDougald. 2006. Regulation of bone mass by Wnt signaling. Journal of Clinical Investigation 116: 1202-1209.

17. E.D Rosen and MacDougald, O.A. 2006. Adipocyte differentiation from the inside out. Nature Reviews - Molecular and Cellular Biology 7: 885-896.

18. Kang, S., V. Dolinksy, and O.A. MacDougald. 2007. Will fatty worms or flies help discover the mechanism of human obesity? In: Genetics of Obesity. Ed. K. Clement and T. Sorensen. pp 143-156.

19. Prestwich, T.C., and O.A. MacDougald. 2007. Wnt/β-catenin signaling in adipogenesis and metabolism. Current Opinion in Cell Biology 19: 612-617.

20. MacDougald, O.A., and C.F. Burant. 2007. The rapidly expanding family of adipokines. Cell Metabolism 6: 159-161.

 

 

 

 
 
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